double superresolution rotary confocal microscope nikon ti2-e Search Results


csu w1 sora superresolution spinning disk confocal  (Yokogawa Electric)
99
Yokogawa Electric csu w1 sora superresolution spinning disk confocal
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Csu W1 Sora Superresolution Spinning Disk Confocal, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+superresolution+rotary+confocal+microscope+nikon+ti2-e/CSU-W1+SoRa/pm39915004-232-16-15
Average 99 stars, based on 1 article reviews
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nikon ti2 e microscope  (Nikon)
99
Nikon nikon ti2 e microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Nikon Ti2 E Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+superresolution+rotary+confocal+microscope+nikon+ti2-e/ECLIPSE+Ti2/pm42834050-774-18-18
Average 99 stars, based on 1 article reviews
nikon ti2 e microscope - by Bioz Stars, 2026-10
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superresolution re-scanning confocal microscopy system rcm-nir  (confocal nl)
90
confocal nl superresolution re-scanning confocal microscopy system rcm-nir
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Superresolution Re Scanning Confocal Microscopy System Rcm Nir, supplied by confocal nl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+superresolution+rotary+confocal+microscope+nikon+ti2-e/super+resolution+re+scan+confocal+microscopy+system+rcm+vis+unit/pm39002468-53-9-19
Average 90 stars, based on 1 article reviews
superresolution re-scanning confocal microscopy system rcm-nir - by Bioz Stars, 2026-10
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orca-flash 4.0 scmos camera  (Hamamatsu)
90
Hamamatsu orca-flash 4.0 scmos camera
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Orca Flash 4.0 Scmos Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+superresolution+rotary+confocal+microscope+nikon+ti2-e/orca+flash+4+0+camera/pm36303461__ja2c10495_si_001-54-31-35
Average 90 stars, based on 1 article reviews
orca-flash 4.0 scmos camera - by Bioz Stars, 2026-10
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nikon imaging software  (Nikon)
99
Nikon nikon imaging software
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Nikon Imaging Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+superresolution+rotary+confocal+microscope+nikon+ti2-e/NIS-Elements/pm39106306-243-54-54
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Image Search Results


Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa CSU-W1 SoRa super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.

Journal: Journal for immunotherapy of cancer

Article Title: Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality.

doi: 10.1136/jitc-2024-010763

Figure Lengend Snippet: Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa CSU-W1 SoRa super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.

Article Snippet: Images were captured with a Nikon Eclipse Ti2- E fully motorized inverted microscope with a Yokogawa CSU- W1 SoRa superresolution spinning disk confocal and 20×/0.45 s Plan Fluor objective.

Techniques: Expressing, Staining, Microscopy, Co-Culture Assay, Control, Single Cell Tracking, Inverted Microscopy, Imaging